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Briefly, zinc diethyldithiocarbamate (final concentration: 1 mM) was added to the sample to inhibit the activity of Cu/Zn-SOD and extracellular SOD ( Detection of Cellular and Mitochondrial ROS Intracellular ROS level was detected with CM-H 2 DCFDA (Shah et al., 2013
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In a randomized, placebo-controlled intervention study with more than 900 older participants experiencing psychological distress, daily supplementation with folic acid (400 μg) and vitamin B 12 (100 μg) for two years did not reduce the occurrence of symptoms of depression despite significantly improving blood folate, vitamin B 12 , and homocysteine levels compared to placebo (109)
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