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Excessive reactive oxygen species (ROS) in the cornea not only elevate the production of O 2 − in mitochondria and impair mitochondrial function [120], but also trigger the release of inflammatory cytokines and recruitment of macrophages

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After 72 h treatment, cells were harvested, trypsinized, washed with cold PBS, and stained with 1X binding buffer containing annexin V-FITC solution and PI