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Then cDNA was synthesized using SuperScript TM IV VILO TM Master Mix (Invitrogen, Waltham, MA, United States) and diluted 10–1 by DNase/RNase-Free Distilled Water (Invitrogen)

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GHK-Cu Titration Protocols: A Biomarker-Informed Framework Titration strategies for GHK-Cu are individualized, typically starting at lower doses and increasing over 4–12 weeks based on clinical response, injection site tolerance, and biomarker trends