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A randomized controlled trial in 167 older adults (≥50 years) found two-year supplementation with vitamin B 12 (10 μg/day), along with folic acid (200 μg/day), vitamin B6 (10 mg/day), riboflavin (5 mg/day), and vitamin D (10 μg/day), had no effect on bone mineral density (total hip, femoral neck, or lumbar spine) compared to the control group only receiving vitamin D (10 μg/day ) (116)