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Relative to the wild-type enzyme, mutation of residue 15 to serine or alanine in UGT74F2 did not lessen the specific activity for SGE formation, but did increase the formation of SAG
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We treated hepatocytes with auranofin, which inhibits redox-active selenoproteins, including thioredoxin reductases and, at the dose used here, glutathione peroxidases 1, 2, 3, 4, and 6 (Chaudiere and Tappel, 1984, Roberts and Shaw, 1998, Scarbrough et al., 2012)