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Cells were maintained in high glucose DMEM (HyClone, Logan, UT) with 1 mM pyruvate and 4 mM glutamine media supplemented with 10% FBS (Equitech Bio, Lewisville, TX), and Pen/Strep in monolayers in 100 mm Greiner tissue culture dishes (Greiner, Orlando, FL) at standard cell culture conditions (5% CO 2 , 95% air)